【作者】趙微微; 李海波; 付立忠; 吳學謙; 許修宏; 賀亮; 程俊文; 魏海龍; 吳慶其
【Author】ZHAO Weiwei1; LI Haibo 2; FU Lizhong2; 3; WU Xueqian 2; 3; XU Xiuhong1; HE Liang 2; CHENG Junwen 2; WEI Hailong2; 3; WU Qingqi2; 31Northeast Agricultural University; Harbin; Heilongjiang 150030; China; 2ZhejiangForestry Academy; Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of ForestResource; Hangzhou; Zhejiang 310023; China; 3Lishui Edible Fungal Research and DevelopmentCenter; Lishui; Zhejiang 323000; China
【作者機構】東北農(nóng)業(yè)大學; 浙江省林業(yè)科學研究院浙江省森林資源生物與化學利用重點實驗室; 麗水市食用菌研究開發(fā)中心
【摘要】 利用前期研究開發(fā)的10個SCAR分子標記對47個香菇主栽菌株的基因組DNA進行擴增分析。基于SCAR標記在檢測菌株中的存在與缺失差異,7個香菇菌株被首先鑒別出來,其余40個菌株被分成11個小組,其中一些形態(tài)特征極為相近的菌株,如L241與L241-4、閩豐1號與L12、Cr02與7402等被相互辨別。
【Abstract】 Forty-seven Lentinula edodes strains that are presently cultivated in China on a large scale were collected and analyzed with 10 previously developed stable sequence characterized amplified region(SCAR) molecular markers.Based on detected differences in SCAR phenotypes,seven individual L.edodes strains were identified,and the remaining 40 strains were separated into 11 distinct groups.Our data provide the first molecular-based evidence confirming the distinction between closely related L.edodes strains having similar morphological features,including strains L241 and L241-4,Minfeng-1 and L12,and Cr02 and 7402.
【關鍵詞】 香菇; 主栽品種; SCAR標記; 分子鑒定; 食用菌
【Key words】 Lentinula edodes strains; major cultivars; SCAR molecular marker; molecular identification; edible mushroom
【基金】“十一五”國家科技支撐計劃課題“人工栽培食用菌優(yōu)質新品種選育研究”(編號:2008BADA1B02);; 浙江省創(chuàng)新團隊建設與人才培養(yǎng)項目“現(xiàn)代食用菌技術創(chuàng)新團隊建設”(編號:2009F20019);; 浙江省農(nóng)業(yè)科技成果轉化資金項目“專用型香菇新品種推廣與種性安全快速鑒定技術示范應用”的部分研究內(nèi)容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-002
【分類號】S646.12